Anti-Halo tag, AlpSdAbs® VHH

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Halo tag
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:

Anti-Halo tag, AlpSdAbs® VHH is designed for detecting Halo tag fusion proteins specifically. Anti-Halo tag, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-Halo tag, AlpSdAbs® VHH detects the Halo tag selectively, no reactivity with other proteins.

Immunogen: Halo tag fused KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Halo tag
Cross-Reactivity: Highly selective for Halo tag sequence
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

The protein tag(Halo tag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (Halo tag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Due to the single-chain nature of sdAbs and their increased sensitivity and higher affinity to Halo tag fusion proteins, Anti-Halo Tag, AlpHcAbs® Rabbit antibody can be used to detect the Halo tag selectively, with high sensitivity and negligible non-specific background.

性能 >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.