Smart Boosters are an innovative research tools developed by AlpVHHs. Smart Boosters involve the site-specific conjugation of fluorescent probes to nanobodies, which does not affect the antibody activity while clearly defining the labeling ratio for quantification. Due to the small size of nanobodies (15 KD), Smart Boosters can achieve a higher labeling density and image resolution:

Advantages

• Better tissue penetration.

• Minimal error: with an epitope labeling displacement of less than 2 nm.

• GFP and RFP-Boosters can stabilize, retain, and enhance the signals of fluorescent proteins.

• It is easy to access and label in dense cell/organelle environments.

• It can be used for quantification.

Applications

• Immunofluorescence, FACS.

• Super-resolution microscopy, confocal microscopy, and epifluorescence microscopy.

• Light-sheet microscopy imaging, including tissue clearing, organ, and animal (vDISCO) staining.