High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Human IgA, AlpHcAbs® Goat antibody(Biotin) is designed for detecting human IgA specifically. Anti-Human IgA, AlpHcAbs® Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgA coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgA, AlpHcAbs® Goat antibody(Biotin) reacts with human IgA selectively.
Immunogen: Human IgA
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Biotin
Specificity: Human IgA
Cross-Reactivity: Does not bind to human IgG, IgD, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgA is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
ELISA： 1:5000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
