Nano-Secondary Antibody

Nano-Secondary Antibody are a series of products toward the immunoglobulins from different species(mouse, rabbit, human, cynomolgus, rat, goat, alpaca,etc.)

Based on single domain antibody(Nanobody, VHH) derived from the variable regions of heavy chain of Alpaca pacous, AlpVHHs offers a range of antibody products, including VHH, Biotin or HRP labeled VHH, fluorescent molecular labeled VHH, or resins(agarose or magnetic bead) labeled VHH. Those products are useful and efficient for Western Blotting(WB), Enzyme Linked Immunosorbent Assay(ELISA), Immunoprecipitation(IP), Immunofluorescence(IF), Immunocytochemistry(ICC), Flow Cytometry(FCM), Immunohistochemistry(IHC), purification, etc.  

VHH-The smallest antibody

Camelids (alpaca, llama, camel, vicuna and guanaco) can produce two non-canonical immunoglobulins alongside the conventional IgG1. IgG2 and IgG3 are known as heavy chain only antibodies. They have single domain binding sites (VHH), and do not contain CH1 domains or light chains (Figure 1).

The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. VHH contains longer CDRs compared to conventional antibodies, and provide the antibody access to recessed antigenic sites not available to canonical antibodies.

Thus VHH is considered of great value for research, medical diagnostics, therapeutics, biosensors, crystallization partners, and detection of small molecules.

Features of VHH antibodies

Targeting

The small size and long CDR3 loop of the VHH antibodies allows better access to cryptic epitopes compared to conventional antibodies.

Stability

VHH antibodies have good solubility and are stable to heat, proteases and pH extremes.

Production

VHH antibodies are easy to clone and modify. They can be recombinant produced at high levels in bacterial, yeast, and mammalian expression systems. Consistent and reproducible results can be guaranteed.

Modification

VHH domain is small and stable, so VHH antibodies can be designed in a variety of structural formats. VHH can act as a framework for recombinant Fc fused antibody production or creating new antibodies by combination two or more VHHs.

Strict validation

All the antibodies are verified by multiple test, and specificity is guaranteed. All the antibodies are selected with high affinity (nM level), and the applications of the antibodies are clear. Using the antibodies provided by AlpVHHs, The accuracy of experiment is guaranteed.

Stable supply

The molecular sequence of antibody is defined, and stable cell lines for recombinant expression have been constructed. Dependable and stable supply is guaranteed. Lot-to-lot difference can be eliminated by stable production and supply.

Superior Performance

All the products are recombinant expressed and tested before delivering to customer. Consistent and reproducible results are guaranteed.

Special Technology

VHH owns the feature of smaller size with better and easier modification. Specific modification had been designed to produce different kinds of products to meet better performance.

Ethical issues

Limited chemical component medium for recombinant expression, no animal serum or other animal derived components are used.

AlpVHHs has focused on the research and development of single domain antibody(Nanobody, VHH) for many years. We are committed to provide more efficient, more stable and more convenient antibody reagents for scientific research, in vitro diagnosis and drug development.