Vector backbone:pYD1
Vector type:Yeast Expression
Vector length: 5179 bp
Tag:HA tag
Promoter: GAL1
Resistance: Amp+
Description:
The vector pYDC2 is constructed on the basis of plasmid pYD1, and the C-terminus of the display protein is connected to the anchor protein, which can more fully display the N-terminus of the exposed display protein, and has an HA tag for convenient detection of display signals.
Background:
Yeast display antibody library technology is one of the most prominent research advances in the field of antibody engineering in recent years. This technology mainly displays antibody molecules on the surface of yeast cells, uses target antigen molecules to screen yeast cells expressing specific antibody molecules, and uses genetic engineering methods to express and subsequently identify the functions of antibodies, thereby obtaining functional molecules.