High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-turboGFP, AlpSdAbs® VHH is designed for detecting turboGFP fusion proteins specifically. Anti-turboGFP, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-turboGFP, AlpSdAbs® VHH detects the turboGFP selectively, no reactivity with other proteins.
Immunogen: TurboGFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: TurboGFP
Cross-Reactivity: No cross-reactivity with CopGFP, jellyfish GFP and derivatives
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata. It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies do not bind to TurboGFP. TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
