High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-mNeongreen, AlpSdAbs® VHH is designed for detecting mNeongreen fusion proteins specifically. Anti-mNeongreen, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-mNeongreen, AlpSdAbs® VHH detects the mNeongreen selectively, no reactivity with other proteins.
Immunogen: mNeongreen fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: mNeongreen
Cross-Reactivity: Highly selective for mNeongreen
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
mNeongreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins.
WB: 1:1,000-1:5000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
