Anti-TagFP, AlpSdAbs® VHH

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                TagFP
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-TagFP, AlpSdAbs® VHH is designed for detecting TagFP fusion proteins specifically. Anti-TagFP, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-TagFP, AlpSdAbs® VHH detects the TagFP selectively, no reactivity with other proteins.

Immunogen: mNeogreen fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: TagFP(TagRFP/TagBFP)
Cross-Reactivity: Highly selective for TagRFP/TagBFP. Does not cross-react with common GFP or dsRed derivatives.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. For biochemical analysis including mass spectrometry and enzymeactivity measurements.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.