Anti-RFP, AlpSdAbs® VHH - 临界点生物

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                RFP
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:

Anti-RFP, AlpSdAbs® VHH is designed for detecting RFP fusion proteins specifically. Anti-RFP, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-RFP, AlpSdAbs® VHH detects the RFP selectively, no reactivity with other proteins.

Immunogen: RFP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mScarlet, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

性能 >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1:100-1:500

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.