Anti-MBP, AlpSdAbs® VHH(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                MBP
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:

Anti-MBP, AlpSdAbs® VHH(HRP) is designed for detecting MBP fusion proteins. Anti-MBP, AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant, single domain antibody to MBP coupled to HRP, and Anti-MBP, AlpSdAbs® VHH(HRP) detects the MBP selectively, no reactivity with other proteins.

Immunogen: MBP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.

性能 >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.