Anti-His tag, AlpSdAbs® VHH

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                His tag,His tag
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Summary >

Description:

Anti-His tag, AlpSdAbs® VHH is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-His tag, AlpSdAbs® VHH detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(A HA tag and one cys were added at the C terminal of the VHH)
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

The His tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein's biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.