High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-GST, AlpSdAbs® VHH is designed for detecting GST fusion proteins specifically. Anti-GST, AlpSdAbs® VHH is based on monoclonal, recombinant, single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-GST, AlpSdAbs® VHH detects the GST selectively, no reactivity with other proteins.
Immunogen: GST fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: GST
Cross-Reactivity: Highly selective for GST
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
