Details and Advantages
Applications:
WB,ELISA,IP
Reactivity:
Human IgG(H+L)
Conjugate:
Biotin
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
>
Description:
Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) is designed for detecting human IgG(H+L) specifically. Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) is based on recombinant single domain antibodies to human IgG(H+L) coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) reacts with the human IgG(H+L) specifically.
Immunogen: Recombinant human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Human IgG(H+L)
Cross-Reactivity: Recognizes human IgG specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) is designed for detecting human IgG(H+L) specifically. Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) is based on recombinant single domain antibodies to human IgG(H+L) coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(H+L), AlpSdAbs® VHH(Biotin) reacts with the human IgG(H+L) specifically.
Immunogen: Recombinant human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Human IgG(H+L)
Cross-Reactivity: Recognizes human IgG specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
性能
>
Flow Cyt: 1:200-1:2000
ELISA: 1:10,000-1:50,000
WB: 1:10,000-1:50,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ELISA: 1:10,000-1:50,000
WB: 1:10,000-1:50,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
