Anti-GST, AlpSdAbs® VHH(Biotin)

Details and Advantages

                                                Applications:
                                                ELISA,Purification,BLI,SPR
                                            
                                                Reactivity:
                                                GST
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:

Anti-GST, AlpSdAbs® VHH(Biotin) is designed for detecting GST fusion proteins. Anti-GST, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to GST coupled to Biotin, and Anti-GST, AlpSdAbs® VHH(Biotin) detects the GST selectively, no reactivity with other proteins.

Immunogen: GST fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: GST
Cross-Reactivity: Highly selective for GST
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.

性能 >

WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000

IP: 1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.