High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-mNeongreen, AlpHcAbs® Rabbit antibody is designed for detecting mNeongreen fusion protein specifically. Anti-mNeongreen, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-mNeongreen, AlpHcAbs® Rabbit antibody is useful for detecting mNeongreen fusion proteins with high sensitivity.
Immunogen: mNeongreen
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: mNeongreen
Cross-Reactivity: Highly selective for mNeongreen
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
mNeongreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins.
WB: 1:1,000-1:5,000
ELISA: 1:5,000-1:20,000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
