High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-TagFP, AlpHcAbs® Rabbit antibody is designed for detecting TagFP fusion protein specifically. Anti-TagFP, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-TagFP, AlpHcAbs® Rabbit antibody is useful for detecting TagFP fusion proteins with high sensitivity.
Immunogen: TagFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: TagFP(TagRFP/TagBFP)
Cross-Reactivity: Highly selective for TagRFP/TagBFP. Does not cross-react with common GFP or dsRed derivatives.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. For biochemical analysis including mass spectrometry and enzymeactivity measurements.
WB: 1:5,000-1:20,000
ELISA: 1:5,000-1:20,000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
