Details and Advantages
Applications:
IP,ICC/IF,Flow Cyt,WB,ELISA
Reactivity:
StayGold
Conjugate:
Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-StayGold, AlpHcAbs® Rabbit antibody is designed for detecting StayGold fusion protein specifically. Anti-StayGold, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-StayGold, AlpHcAbs® Rabbit antibody reacts with the StayGold selectively, no reactivity with other proteins.
Immunogen: StayGold fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)
Conjugate: Unconjugated
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imagingUsing antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-StayGold, AlpHcAbs® Rabbit antibody is designed for detecting StayGold fusion protein specifically. Anti-StayGold, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-StayGold, AlpHcAbs® Rabbit antibody reacts with the StayGold selectively, no reactivity with other proteins.
Immunogen: StayGold fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)
Conjugate: Unconjugated
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)
Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imagingUsing antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Performance
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WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
ICC/IF: 1:100-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10^6 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ELISA: 1:5,000-1:20000
ICC/IF: 1:100-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10^6 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
