High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-MBP, AlpHcAbs® Rabbit antibody is designed for detecting MBP fusion protein specifically. Anti-MBP, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-MBP, AlpHcAbs® Rabbit antibody is useful for detecting MBP fusion proteins with high sensitivity.
Immunogen: MBP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.
WB: 1:5,000-1:20,000
ELISA: 1:5,000-1:20,000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
