High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-GST, AlpHcAbs® Rabbit antibody is designed for detecting GST fusion proteins. Anti-GST, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-GST, AlpHcAbs® Rabbit antibody detects the GST selectively, no reactivity with other proteins.
Immunogen: GST fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: GST
Cross-Reactivity: No cross-reactivity with other proteins
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: PBS pH=7.510mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: store at –20 °C. Avoid freeze / thaw cycle
Background:
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade. GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
WB: 1:5000-1:20,000
ELISA: 1:5,000-20,000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
