Details and Advantages
Applications:
WB,ELISA,IP
Reactivity:
Sheep IgG
Conjugate:
Biotin
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-Sheep IgG(H+L), AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting Sheep IgG specifically. Anti-Sheep IgG(H+L), AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to Sheep IgG coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Sheep IgG(H+L), AlpHcAbs® Rabbit antibody(Biotin) reacts with Sheep IgG selectively, no reactivity with mouse, human, cynomolgus, rat, rabbit IgG.
Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Conjugate: Biotin-SP (long spacer)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, rabbit IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-Sheep IgG(H+L), AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting Sheep IgG specifically. Anti-Sheep IgG(H+L), AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to Sheep IgG coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Sheep IgG(H+L), AlpHcAbs® Rabbit antibody(Biotin) reacts with Sheep IgG selectively, no reactivity with mouse, human, cynomolgus, rat, rabbit IgG.
Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Conjugate: Biotin-SP (long spacer)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, rabbit IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
性能
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ELISA：1:5000-1:20000
WB: 1:5000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:5000-1:20000
IP: 1-2ug/sample
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
