·High lot-to-lot consistency
·Increased sensitivity and higher affinity
·Animal-free production
Description:
Anti-Mouse IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) is designed for detecting mouse IgG Fcγ fragment(including mouse IgG1, IgG2a, IgG2b, IgG3) specifically. Anti-Mouse IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG Fcγ fragment coupled to Biotin, and Anti-Mouse IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(Biotin) reacts with the Fc fragment of mouse IgG heavy chain but not with the Fab portion of mouse IgG.
Immunogen: Recombinant mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Conjugate: Biotin-SP (long spacer)
Specificity: Mouse IgG(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Affinity: ＜1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
ELISA: 1:10000-1:50000
WB: 1:10000-1:50000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
