Anti-Llama IgG(H+L), AlpHcAbs® Goat antibody(FITC)

Details and Advantages

                                                Applications:
                                                ICC/IF,IHC
                                            
                                                Reactivity:
                                                Llama IgG(H+L)
                                            
                                                Conjugate:
                                                FITC
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity

概述 >

Description:
Anti-Llama IgG(H+L), AlpHcAbs® Goat antibody(FITC) is designed for detecting Llama IgG(H+L) specifically. Based on immunoelectrophoresis and/or ELISA, Anti-Llama IgG(H+L), AlpHcAbs® Goat antibody(FITC) reacts with Llama IgG heavy chain and light chain selectively.

Immunogen: Llama immunoglobulins
Host: Goat
Isotype: Goat IgG
Conjugate: FITC(Ex=493nm, Em=528nm), 3 moles FITC per mole IgG
Specificity: Llama IgG(H+L)
Cross-Reactivity: Llama IgG and with light chains common to other Llama immunoglobulins(such as IgA, IgM). No was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Purity: Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS pH 7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light

Background:
The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains; each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb).
Llama heavy chain only antibodies provide a framework for engineering recombinant antibodies. The monomeric variable domain (VHH) of heavy chain only camelid IgG2 and IgG3 provides a 12-15 kDa domain which is easy to clone and produce at high levels in recombinant systems, is stable to heat and pH extremes, and generally, has good solubility.

性能 >

ICC/IF: 1:200-1:2000
IHC-P: 1:200-1:2000
Almost it can be used for VHH that come from Llama

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.