Anti-Human kappa, AlpHcAbs® Goat antibody(iFluor594)

Details and Advantages

                                                Applications:
                                                WB,ICC/IF,ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                Human kappa
                                            
                                                Conjugate:
                                                iFluor594
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Human kappa, AlpHcAbs® Goat antibody(iFluor594) is designed for detecting human IgG kappa light chain specifically. Anti-Human kappa, AlpHcAbs® Goat antibody(iFluor594) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgG kappa light chain coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-Human kappa, AlpHcAbs® Goat antibody(iFluor594) reacts with human IgG kappa light chain selectively, no reactivity with rabbit, mouse, rat, goat IgG.

Immunogen: Fab region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: iFluor594(Ex: 590nm, Em: 617nm), 3 moles iFluor594 per mole IgG
Specificity: Human IgG kappa chain
Cross-Reactivity: Cross-react with cynomolgus IgG, No cross-reactivity with rabbit, mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

性能 >

ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.