High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Human IgG(Fab Fragment specific), AlpHcAbs® Goat antibody is designed for detecting human IgG fab fragment specifically. Anti-Human IgG(Fab Fragment specific), AlpHcAbs® Goat antibody is monovalent, recombinant single domain antibody fused to goat IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(Fab Fragment specific), AlpHcAbs® Goat antibody reacts with human IgG fab fragment selectively, no reactivity with rabbit, mouse, rat, goat IgG.
Immunogen: Fab region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: Human IgG fab fragment
Cross-Reactivity: Cross-react with cynomolgus IgG, No cross-reactivity with rabbit, mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Background:
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
