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Anti-Human IgD, AlpHcAbs® Goat antibody(HRP)

Details and Advantages
Applications: ELISA
Reactivity: Human IgD
Conjugate: HRP
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >
Description:
Anti-Human IgD, AlpHcAbs® Goat antibody(HRP) is designed for detecting human IgD specifically. Anti-Human IgD, AlpHcAbs® Goat antibody(HRP) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to human IgD coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgD, AlpHcAbs® Goat antibody(HRP) reacts with human IgD selectively.

Immunogen: Human IgD
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: HRP
Specificity: Human IgD
Cross-Reactivity: Does not bind to human IgG, IgA, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Performance >
ELISA: 1:5000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.