Details and Advantages
Applications:
ICC/IF,Flow Cyt,WB,ELISA,IP
Reactivity:
Biotin
Conjugate:
Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-Biotin, AlpHcAbs® Rabbit antibody is designed for detecting biotin or biotinylated protein specifically. Anti-Biotin, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Biotin, AlpHcAbs® Rabbit antibody reacts with biotin or biotinylated protein selectively, no reactivity with other antigen.
Immunogen: Biotin conjugated KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: Biotin and biotinylated antibody, protein, peptides, oligonucleotides or solid matrices.
Cross-Reactivity: No cross-reactivity with other antigen.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
Biotin is widely conjugated to proteins and antibodies for biochemical assays. Avidin (streptavidin)-biotin system is commonly used for many immunoassays such as ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, and Immunohistochemistry. Anti-Biotin antibody is a better alternative to avidins to minimize background and maximize signal intensity.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-Biotin, AlpHcAbs® Rabbit antibody is designed for detecting biotin or biotinylated protein specifically. Anti-Biotin, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Biotin, AlpHcAbs® Rabbit antibody reacts with biotin or biotinylated protein selectively, no reactivity with other antigen.
Immunogen: Biotin conjugated KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: Biotin and biotinylated antibody, protein, peptides, oligonucleotides or solid matrices.
Cross-Reactivity: No cross-reactivity with other antigen.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
Biotin is widely conjugated to proteins and antibodies for biochemical assays. Avidin (streptavidin)-biotin system is commonly used for many immunoassays such as ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, and Immunohistochemistry. Anti-Biotin antibody is a better alternative to avidins to minimize background and maximize signal intensity.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Performance
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ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
ICC/IF: 1:100-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10^6 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:5000-1:20000
ICC/IF: 1:100-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10^6 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
