High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Alpaca IgG2b+2c, AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting Alpaca IgG2b+2c specifically. Based on immunoelectrophoresis and/or ELISA, Anti-Alpaca IgG2b+2c, AlpHcAbs® Rabbit antibody(Biotin) reacts with Alpaca IgG2b+2c selectively, no cross-reactivity with Alpaca IgG1.
Immunogen: Recombinant Fc region of alpaca IgG2b/2c
Host: Rabbit
Isotype: Rabbit IgG
Conjugate: Biotin-SP (long spacer)
Specificity: Alpaca IgG2b+2c(Fcγ Fragment specific)
Cross-Reactivity: No cross-reactivity with Alpaca IgG1
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Background:
The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains; each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb). Alpaca is also called Vicugna pacos. Alpaca IgG contains IgG1a, IgG1b, IgG2b, IgG2c and IgG3.
ELISA：1:4000-1:10000
WB: 1:4000-1:10000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
