tdTomato Nanoselector Agarose

Details and Advantages

                                                Applications:
                                                IP,CHIP,MS
                                            

                                                Reactivity:
                                                tdTomato
                                            

                                                Conjugate:
                                                Agarose
                                            

                                                Advantages:
                                                Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)

                                            

概述 >

Description:
tdTomato Nanoselector Agarose has been specifically designed to bind tdTomato-fusion proteins. tdTomato Nanoselector Agarose is based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Agarose beads. tdTomato Nanoselector Agarose is an ideal tool to isolate or purify tdTomato-fusion proteins fast and efficiently.

Ligand: Anti-tdTomato single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm
Reactivity: tdTomato
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant tdTomato.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol

Background:
The tandem dimer Tomato (tdTomato) is a genetically improved version of Discosoma sp. fluorescent protein (dsRed). tdTomato is an extremely bright red fluorescent protein, being one of the brightest red-shifted fluorescent proteins available and six times brighter than eGFP. One of the main advantages of fluorescent proteins is that they do not require substrates or reagents for detection, however most of them do need oxygen for the formation of their chromophores. Besides, fluorescent proteins can be measured without lysing cells, whereas this is difficult for luciferases. One of the biggest disadvantages of fluorescent reporters is that they need to overcome cellular autofluorescence. However, in general cellular autofluorescence is lower for the emission wavelength of the red channel, favoring a red fluorescent protein such as tdTomato.
For biochemical analysis including mass spectrometry and enzyme activity measurements these tdTomato-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the tdTomato Nanoselector Agarose. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. tdTomato Selector is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.

性能 >

Immunoprecipitation (IP)/Co-IP
Mass spectrometry (MS)
Enzyme activity measurements