· Consistent and reproducible results;
· No heavy & light antibody chains Extraordinarybinding,even under harsh conditions;
· High affinity and Short incubation (5-30min)
Description:
MBP Nanoselector Magnetic beads have been specifically designed to bind MBP-fusion proteins. MBP Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. MBP Nanoselector Magnetic beads are ideal tools to isolate or purify MBP-fusion proteins fast and efficiently.
Ligand: Anti-MBP single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 2.8µm
Reactivity: Recognizes MBP fusion protein selectively
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant MBP.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
