· Consistent and reproducible results;
· No heavy & light antibody chains Extraordinarybinding,even under harsh conditions;
· High affinity and Short incubation (5-30min)
Description:
GST Nanoselector Magnetic beads have been specifically designed to bind GST-fusion proteins. GST Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. GST Nanoselector Magnetic beads are ideal tools to isolate or purify GST-fusion proteins fast and efficiently.
Ligand: Anti-GST single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 2.8µm
Reactivity: Recognizes GST fusion protein selectively
Binding Capacity: High binding capacity, 10 µL slurry bind about 25 µg of recombinant GST.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 50 % slurry in PBS containing 20 % Ethanol
Background:
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay. This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest. Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein. GST Nanoselector Agarose is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
