Anti-Human IgG, AlpSdAbs® VHH(VcPBD ×4)

Details and Advantages
Applications: Internalization Test
Reactivity: Human IgG
Conjugate: VcPBD
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >
Description:
Anti-Human IgG, AlpSdAbs® VHH(VcPBD ×4) is designed for studying on the internalization of antibodies. Anti-Human IgG, AlpSdAbs® VHH(VcPBD ×4) is based on monoclonal, recombinant single domain antibody to human IgG Fc coupled to VcPBD. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG, AlpSdAbs® VHH(VcPBD ×4) reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG. Anti-Human IgG, AlpSdAbs® VHH(VcPBD ×4) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.

Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: VcPBD(2 moles VcPBD per mole VHH)
Specificity: Human IgG(Fcγ fragment specific), including IgG1, IgG2, IgG3, IgG4 and their mutations(LALA or YTE)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance >
Antibody Internalization Test: 2ug per 10ug test antibody(molar ratio=2:1).

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.