Details and Advantages
Applications:
WB,ELISA,ICC/IF,Flow Cyt
Reactivity:
Sheep IgG
Conjugate:
iFluor647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor647) is designed for detecting sheep IgG specifically. Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor647) is based on monovalent, recombinant single domain antibody to sheep IgG coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor647) reacts with sheep IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.
Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex: 652nm, Em: 668nm)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor647) is designed for detecting sheep IgG specifically. Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor647) is based on monovalent, recombinant single domain antibody to sheep IgG coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Sheep IgG(H+L), AlpSdAbs® VHH(iFluor647) reacts with sheep IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.
Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex: 652nm, Em: 668nm)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
性能
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ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
