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Anti-Sheep IgG(H+L), AlpSdAbs® VHH

Details and Advantages
Applications: WB,ELISA
Reactivity: Sheep IgG
Conjugate: Unconjugated
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

概述 >
Description:
Anti-Sheep IgG(H+L), AlpSdAbs® VHH is designed for detecting sheep IgG(H+L) specifically. Anti-Sheep IgG(H+L), AlpSdAbs® VHH is monovalent, recombinant single domain antibody derived from the variable regions of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Sheep IgG(H+L), AlpSdAbs® VHH detects sheep IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.

Immunogen: Sheep IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Sheep IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
性能 >
ELISA:1:5000-1:20000
WB: 1:5000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.