Anti-Mouse IgG kappa, AlpSdAbs® VHH

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Mouse kappa
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                ·High lot-to-lot consistency
·Increased sensitivity and higher affinity
·Animal-free production

Summary >

Description:

Anti-Mouse IgG kappa, AlpSdAbs® VHH is designed for detecting mouse IgG kappa chain specifically. Anti-Mouse IgG kappa, AlpSdAbs® VHH is monovalent, recombinant single domain antibody derived from the variable regions of heavy chain of Alpaca pacous, and Anti-Mouse IgG kappa, AlpSdAbs® VHH reacts with the mouse IgG kappa chain selectively, no reactivity with the mouse IgG lambda chain or heavy chain of mouse IgG.

Immunogen: Fab region of Mouse IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Mouse IgG kappa chain
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Performance >

ELISA: 1:5000-1:20000
WB: 1:5000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.