Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647)

Details and Advantages

                                                Applications:
                                                WB,ICC/IF,ELISA,Flow Cyt
                                            
                                                Reactivity:
                                                Mouse IgG3(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                iFluor647
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is designed for detecting mouse IgG3 Fcγ fragment specifically, and Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is useful for super-resolution microscopy. Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is based on recombinant single domain antibody to mouse IgG3 Fc coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG3(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) reacts with the Fc fragment of mouse IgG3 selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Immunogen: Recombinant Fc region of mouse IgG3
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex:651nm, Em:667nm)
Specificity: Mouse IgG3(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2a/2b, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH.
The smaller size of the VHH decreases linkage error and increases staining accuracy effectively. Standard immunodetection approaches use typically a primary antibody (1.Ab) which binds the protein of interest (POI) and a secondary antibody (2.Ab) that binds to the 1.Ab and carries a detection element. The complex formed by the primary antibody and the secondary antibody (1.Ab–2.Ab) is widely used because it is a cost effective and flexible approach since only the 2.Abs need to be coupled to the detection element. However, the use of this complex carries some relevant limitations. The 1.Ab–2.Ab can measure up to 30 nm, leading to a large distance between the targeted molecule and the detection element, causing the so called “linkage” or “displacement” error. While this might not influence the results in some applications (e.g. epifluorescence, ELISA or FACS), it is of major relevance for super-resolution microscopy techniques where the localization precision can be as high as 1 nm. The linkage error can be reduced by using directly labelled small affinity probes like camelid single domain antibodies (sdAbs) also known as nanobodies (Nbs), which have sizes below 3 nm.

性能 >

Flow Cyt: 1:200-1:2000
ICC/IF： 1:200-1:2000
ELISA： 1:10000-1:50000
WB: 1:10000-1:50000
Super-resolution microscopy

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.