Anti-Mouse IgG2a(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Mouse IgG2a(Fcγ Fragment specific)
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                ·High lot-to-lot consistency
·Increased sensitivity and higher affinity
·Animal-free production

Summary >

Description:

Anti-Mouse1 IgG2a(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is designed for detecting the Fc region of mouse IgG2a. Anti-Mouse IgG2a(Fcγ fragment specific), AlpSdAbs® VHH(HRP) is based on monovalent, recombinant single domain antibodies to mouse IgG2a(Fcγ fragment specific) coupled to HRP, and the Anti-Mouse IgG2a(Fcγ fragment specific), AlpSdAbs® VHH(HRP) detects the Fc region of mouse IgG2a selectively, no reactivity with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.

Immunogen: Recombinant Fc region of mouse IgG2a
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Mouse IgG2a(Fcγ fragment specific)
Cross-Reactivity: No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
Affinity: ＜1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light, Stable for 12 months at -20°C

Performance >

ELISA: 1:10000-1:50000
WB: 1:10000-1:50000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.