Anti-Human lambda, AlpSdAbs® VHH(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Human lambda
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Human lambda, AlpSdAbs® VHH(HRP) is designed for detecting human IgG lambda light chain specifically. Anti-Human lambda, AlpSdAbs® VHH(HRP) is based on monovalent, recombinant single domain antibody to human IgG lambda light chain coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Human lambda, AlpSdAbs® VHH(HRP) reacts with human IgG lambda light chain selectively, no reactivity with rabbit, mouse, rat, goat IgG.

Immunogen: Fab(lambda) region of human IgG

Host: Alpaca pacous

Isotype: VHH domain of alpaca IgG2b/2c

Conjugate：HRP

Specificity: Human IgG lambda chain

Cross-Reactivity: Cross-react with cynomolgus IgG, No cross-reactivity with rabbit, mouse, rat, goat IgG

Purity: Recombinant Expression and Affinity purified

Concentration: 1mg/ml

Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light, Stable for 12 months at -20°C

Background:
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

ELISA： 1:5000-1:20000
WB: 1:5000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.