Anti-Human IgA, AlpSdAbs® VHH

Details and Advantages

                                                Applications:
                                                ELISA,IP
                                            
                                                Reactivity:
                                                Human IgA
                                            
                                                Conjugate:
                                                Unconjugated
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-Human IgA, AlpSdAbs® VHH is designed for detecting human IgA specifically. Anti-Human IgA, AlpSdAbs® VHH is monovalent, recombinant single domain antibody derived from the variable regions of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgA, AlpSdAbs® VHH reacts with human IgA selectively, no reactivity with human IgG, IgD, IgM, IgE.

Immunogen: Human IgA
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Unconjugated(6*his tag and one cys were added at the C terminal of the VHH)
Specificity: Human IgA
Cross-Reactivity: Does not bind to human IgG, IgD, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgA is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

ELISA： 1:5000-1:20000
IP: 1-2ug/sample

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.