Details and Advantages
Applications:
ICC/IF,Flow Cyt,WB,ELISA
Reactivity:
Goat IgG(Fcγ Fragment specific)
Conjugate:
iFluor647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is designed for detecting Fc region of goat IgG specifically. Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is based on monovalent, recombinant single domain antibody to goat IgG(Fcγ Fragment specific) coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) reacts with the Fc region of goat IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.
Immunogen: Recombinant Fc region of goat IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex: 652nm, Em: 668nm)
Specificity: Fc region of goat IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Goat antibodies are commonly used in biotechnology. They are used to prepare diagnostic reagents of immunochemical techniques. Goat IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is designed for detecting Fc region of goat IgG specifically. Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) is based on monovalent, recombinant single domain antibody to goat IgG(Fcγ Fragment specific) coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Goat IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647) reacts with the Fc region of goat IgG selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat IgG.
Immunogen: Recombinant Fc region of goat IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex: 652nm, Em: 668nm)
Specificity: Fc region of goat IgG
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
Goat antibodies are commonly used in biotechnology. They are used to prepare diagnostic reagents of immunochemical techniques. Goat IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
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ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
