Anti-Human IgG, AlpSdAbs® VHH(DT)

Details and Advantages
Applications: Internalization Test
Reactivity: Human IgG
Advantages:

High lot-to-lot consistency

Increased sensitivity and higher affinity

Animal-free production

Summary >

Description:

Anti-Human IgG, AlpSdAbs® VHH(DT) is designed for studying on the internalization of antibodies. Anti-Human IgG, AlpSdAbs® VHH(DT) is based on recombinant single domain antibody to human IgG Fc coupled to diphtheria toxin(DT). Anti-Human IgG, AlpSdAbs® VHH(DT) consists of diphtheria toxin (DT) lacking the receptor-binding domain and the VHH binding for human IgG. Anti-Human IgG, AlpSdAbs® VHH(DT) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs. The molecular weight of Anti-Human IgG, AlpSdAbs® VHH(DT) is about 60 kDa.

 

Immunogen: Recombinant Fc region of human IgG                   

Host: Alpaca pacous

Isotype: VHH domain of alpaca IgG2b/2c  

Conjugate: Diphtheria toxin (DT)   

Specificity: Human IgG(Fcγ fragment specific)

Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG      

Purity: Recombinant Expression and Affinity purified

Concentration: 1mg/ml

Formation: Liquid, 10mM PBS (pH 7.4)

Storage: Store at –20 °C(Avoid freeze / thaw cycles)

 

Background:

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.


Performance >

Antibody Internalization Test: 8ug per 10ug antibody

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.