High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Human IgG, AlpSdAbs® VHH(DT) is designed for studying on the internalization of antibodies. Anti-Human IgG, AlpSdAbs® VHH(DT) is based on recombinant single domain antibody to human IgG Fc coupled to diphtheria toxin(DT). Anti-Human IgG, AlpSdAbs® VHH(DT) consists of diphtheria toxin (DT) lacking the receptor-binding domain and the VHH binding for human IgG. Anti-Human IgG, AlpSdAbs® VHH(DT) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs. The molecular weight of Anti-Human IgG, AlpSdAbs® VHH(DT) is about 60 kDa.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Diphtheria toxin (DT)
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Antibody Internalization Test: 8ug per 10ug antibody
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
