Details and Advantages
Applications:
ELISA,IP
Reactivity:
mGold
Conjugate:
Biotin
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
概述
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Description:
Anti-mGold, AlpSdAbs® VHH(Biotin) is designed for detecting mGold fusion proteins. Anti-mGold, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to mGold coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-mGold, AlpSdAbs® VHH(Biotin) detects the mGold selectively, no reactivity with other proteins.
Immunogen: mGold
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: mGold, mGold2s, mGold2t
Cross-Reactivity: Recognizes GFP and YFP variants.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-mGold, AlpSdAbs® VHH(Biotin) is designed for detecting mGold fusion proteins. Anti-mGold, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to mGold coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-mGold, AlpSdAbs® VHH(Biotin) detects the mGold selectively, no reactivity with other proteins.
Immunogen: mGold
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: mGold, mGold2s, mGold2t
Cross-Reactivity: Recognizes GFP and YFP variants.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
性能
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ELISA: 1:5,000-1:20000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
