Multiomic studies, which involve the integration of DNA, RNA, and protein “omics” datasets from a single cell or sample type, are helping researchers understand complex biological processes in development and disease. CUT&Tag, with its ability to profile multiple histone modifications and other chromatin features alongside transcriptome profiling, has become a key tool in multiomics. Its adaptability when combined with single-cell technologies offers unprecedented insights into the epigenetic landscape and the dynamic interplay between chromatin states and gene expression.

How to obtain more high-quality data from limited and valuable samples or single source samples is an urgent problem that researchers need to solve. CUT&Tag technology currently relies on traditional pAG-Tn5 to achieve data collection for a single or at most 2 or 3 targets, greatly limiting the flux of data collection.

In order to achieve the goal of collecting data from a single sample with multiple targets, AlpVHHs offers the Multi-nanoCUT&Tag. Based on unique nanobody（VHH） technology platform and different nanobodies targeting different IgG from different species or different Tags, CUT&Tag can simultaneously collect data from multiple targets(≥4) according to the researcher's combination by fusing different nanobody with Tn5 enzyme.

At AlpVHHs, we offer a variety of VHH-Tn5 fusion enzymes and corresponding reagents and services.

VHH-Tn5, uncharged(specific to different species)

VHH-Tn5, loaded with standard sequencing adapters

Custom VHH-Tn5,