Anti-His tag, AlpHcAbs® Rabbit antibody(Biotin)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                His tag,
                                            
                                                Conjugate:
                                                Biotin
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:

Anti-His tag, AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 to His tag coupled to Biotin, and Anti-His tag, AlpHcAbs® Rabbit antibody(Biotin) detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein

Host: Alpaca pacous

Isotype: Fab of alpaca IgG1 fused to Rabbit Fc(mutation)

Conjugate: Biotin

Specificity: His tag sequence(HHHHHH)

Cross-Reactivity: Highly selective for His tag sequence

Purity: Recombinant expression and Affinity purified

Concentration: 1mg/ml

Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol

Storage: Store at –20 °C, (Avoid freeze / thaw cycles),

Background:

The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.

Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.