Product Name: c-Myc Peptide
Code: APK052
Peptide: EQKLISEEDL
Assay: ≥95% (HPLC/MS)
Form: Lyophilized powder
Storage Temp: 2-8°C
Preparation Note: Dissolve in TBS as a stock solution(Storage at -20°C) at a concentration of 5 mg/ml.
Note: TBS(50 mMTris-HCl, pH 7.4, with 150 mM NaCl)
Description:
The c-Myc Peptide is a synthetic peptide of 10 amino acid residue(EQKLISEEDL).
Benefits:
After competitive elution of the c-Myc tag fusion expression protein from this product, the eluted sample does not contain the light and heavy chains of the traditional Anti-myc tag antibody, effectively solving the interference issue of light and heavy chains during Western detection after immunoprecipitation. In conventional immunoprecipitation, the c-Myc tag fusion expression protein is usually released into the solution by boiling with the addition of loading buffer, but at this time, the light and heavy chains of the Anti-myc tag antibody also enter the solution and are likely to interfere with subsequent Western detection. The most important thing about peptide elution is to maintain the activity of the elution product.
Recommended working concentration is 100-400μg/ml for elute 3×Flag fusion proteins from the c-Myc affinity gel(Myc tag Nanoselector Agarose, Myc tag Nanoselector Magnetic beads or other Anti-Myc affinity gel).
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antibody density, permeability, etc. The actual dilution used must be determined empirically.
