Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)
Description:
mWassabi Nanoselector Magnetic beads have been specifically designed to bind mWassabi-fusion proteins. mWassabi Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. mWassabi Nanoselector Magnetic beads are ideal tools to isolate or purify mWassabi-fusion proteins fast and efficiently.
Ligand: Anti-mWassabi single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40µm
Reactivity: Highly selective for mWassabi fusion protein
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant mWassabi protein.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300
Background:
Fluorescent proteins are widely used to study protein localization and dynamics. mWasabi is engineered variant of monomeric mTFP1, and it is a bright, monomeric green fluorescent protein. mWasabi is nearly 2-fold brighter than EGFP. And different from many commonly used fluorescent proteins, mWasabi is a true monomer and is most likely to not interfere with function or localization of its fusion partner. So mWasabi can be a direct replacement for EGFP or other GFPs. mWassabi-fusion proteins and their interacting factors can to be isolated fast and efficiently by immunoprecipitation using the mWassabi Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.
Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis
