High lot-to-lot consistency
Description:
Human CD7-PE CAR TEST Kit is designed for detecting antibody expression of CAR related cell specifically. Human CD7-PE CAR TEST Kit is recombinant human CD7 antigen fused to PE. Based on FCM and/or ELISA, CD7-PE CAR TEST Kit reacts with Anti-human CD7 antibody and their chimerism.
Protein Construction：Human CD7(Ala26-Pro180) conjugated to PE
Expression System：CHO
Purity: Recombinant Expression and Affinity purified
Conjugate: PE(Ex: 488nm, Em: 575nm)
Concentration: 0.2mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at 4°C(Avoid freeze / thaw cycles), protect from light
Background:
T-Cell Antigen CD7 is a single-pass type I membrane protein that that belongs to the the immunoglobulin superfamily. Human CD7 is synthesized as a 240 amino acid precursor that contains a 25 amino acid signal sequence and a 215 amino acid mature chain with a Ig-like (immunoglobulin-like) domain. CD7 is normally expressed on all T-lymphocytes, NK-cells, pre-B lymphocytes and pleuripotent hematopoietic stem cells. CD7 plays an essential role in T-cell interactions, T-cell/B-cell interaction during early lymphoid development, T- and NK-cell activation and cytokine production. CD7 has been shown to interact with PIK3R1and SECTM1. However, the function of the CD7 protein in the immune system is still largely unknown.
Flow Cyt: 1:100-1:400
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
