Details and Advantages
Applications:
Internalization Test
Reactivity:
Human IgG
Conjugate:
VcMMAE
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
>
Description:
Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) is designed for studying on the internalization of antibodies. Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) is based on recombinant single domain antibodies to human IgG coupled to VcMMAE. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) reacts with the Fc fragment of human IgG heavy chain and kappa chain of human IgG. Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: VcMMAE(2 moles VcMMAE per mole VHH)
Specificity: Human IgG(H&L), including IgG1, IgG2, IgG3, IgG4 and their mutations(LALA or YTE)
Cross-Reactivity: Recognizes human IgG specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) is designed for studying on the internalization of antibodies. Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) is based on recombinant single domain antibodies to human IgG coupled to VcMMAE. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) reacts with the Fc fragment of human IgG heavy chain and kappa chain of human IgG. Anti-Human IgG, AlpSdAbs® VHH(VcMMAE ×8) is an effective detection tool and can be used as a useful tool for the evaluation of antibody potency prior to ADCs.
Immunogen: Recombinant human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: VcMMAE(2 moles VcMMAE per mole VHH)
Specificity: Human IgG(H&L), including IgG1, IgG2, IgG3, IgG4 and their mutations(LALA or YTE)
Cross-Reactivity: Recognizes human IgG specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 0.5mg/ml
Formation: Liquid, 10mM PBS (pH 7.4)
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Performance
>
Antibody Internalization Test: 4ug per 10ug antibody (molar ratio=4:1).
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
