Western blot-Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(082-201-001)
Lane1-6: different amount(200ng-100ng-50ng-25ng-12.5-6.25ng) of mBaojin was loaded.
First antibody:Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(082-201-001) at 0.1 µg/ml(1:10000)
Secondary antibody：
Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP)(025-401-005)(1:10000)
First antibody:Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(082-201-001) at 0.1 µg/ml(1:10000)
Secondary antibody：
Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP)(025-401-005)(1:10000)
Elisa experiment of different antigen using Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody
The plate was coated with different amounts of StayGold or its variants. 1:10000 dilution of Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody was used as the primary antibody. Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) (025-102-005) was used as the secondary antibody.
Confocal experiment of StayGold-Ensconsin transfected COS-7 using Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody
StayGold-Ensconsin transfected COS-7 cells were stained with Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody (1:500) and Goat Anti-Rabbit IgG Antibody(AF647) was the secondary antibody(1:1000). (This picture is kindly provided by Zhang Lab, School of Life Sciences, Westlake University)
Western Blot-Anti-StayGold, AlpHcAbs® Rabbit antibody
Different amount of reduced StayGold is detected by Anti-StayGold, AlpHcAbs® Rabbit antibody with 1:5000 dilution, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)(025-101-005) is used as the secondary antibody.
A titer ELISA of StayGold-Anti-StayGold, AlpHcAbs® Rabbit antibody
The plate was coated with different amounts of StayGold or its variants. 1:5000 dilution of Anti-StayGold, AlpHcAbs® Rabbit antibody was used as the detection antibody, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)(025-101-005) is used as the secondary antibody.