Lane1-6: different amount(100ng-50ng-25ng-12.5-6.25-3.125ng) of MBP was loaded.
First antibody:Anti-MBP, AlpHcAbs® Rabbit antibody(015-201-001) at 0.1 µg/ml(1:10000)
Secondary antibody：
Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP)(025-401-005)(1:10000)