Myc tag Nanoselector Magnetic beads

Details and Advantages

                                                Applications:
                                                IP,CHIP,MS,Purification
                                            

                                                Reactivity:
                                                Myc tag
                                            

                                                Conjugate:
                                                Magnetic beads
                                            

                                                Advantages:
                                                Consistent and reproducible results
No heavy & light antibody chains
Extraordinary binding, even under harsh conditions
High affinity
Short Incubation (5-30 min)

                                            

概述 >

Description:
Myc tag Nanoselector Magnetic beads have been specifically designed to bind Myc tag-fusion proteins. Myc tag Nanoselector Magnetic beads are based on small high-affinity recombinant single domain antibody covalently coupled to the surface of Magnetic beads. Myc tag Nanoselector Magnetic beads are ideal tools to isolate or purify Myc tag-fusion proteins fast and efficiently.

Ligand: Anti-Myc tag single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm

Reactivity: The Myc tag Nanoselector recognizes the Myc-tag sequence of the fusion protein.
Binding Capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant Myc-MG.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
For biochemical analysis including mass spectrometry and enzyme activity measurements these Myc tag-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the Myc tag Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.

性能 >

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays
ChIP, RIP analysis